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Evidence Guide: MSL975062 - Perform microbiological tests

Student: __________________________________________________

Signature: _________________________________________________

Tips for gathering evidence to demonstrate your skills

The important thing to remember when gathering evidence is that the more evidence the better - that is, the more evidence you gather to demonstrate your skills, the more confident an assessor can be that you have learned the skills not just at one point in time, but are continuing to apply and develop those skills (as opposed to just learning for the test!). Furthermore, one piece of evidence that you collect will not usualy demonstrate all the required criteria for a unit of competency, whereas multiple overlapping pieces of evidence will usually do the trick!

From the Wiki University

 

MSL975062 - Perform microbiological tests

What evidence can you provide to prove your understanding of each of the following citeria?

Receive samples and process associated request forms

  1. Identify specimens and request forms that do not comply with workplace requirements
  2. Record any discrepancies or sample quality issues and indicate action required
  3. Register samples in accordance with workplace procedures, recording details that allow accurate tracking and chain of custody traceable to employee
Identify specimens and request forms that do not comply with workplace requirements

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Record any discrepancies or sample quality issues and indicate action required

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Register samples in accordance with workplace procedures, recording details that allow accurate tracking and chain of custody traceable to employee

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare for safe microbiological work and aseptic applications

  1. Apply principles of aseptic techniques to ensure sample and reagent integrity are maintained
  2. Apply principles of aseptic techniques to ensure personal and environmental safety
  3. Minimise the production and release of aerosols, using biological safety cabinets where necessary
  4. Safely dispose of biohazardous materials and other laboratory wastes in accordance with workplace procedures
Apply principles of aseptic techniques to ensure sample and reagent integrity are maintained

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Apply principles of aseptic techniques to ensure personal and environmental safety

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Minimise the production and release of aerosols, using biological safety cabinets where necessary

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Safely dispose of biohazardous materials and other laboratory wastes in accordance with workplace procedures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Process samples for direct examination

  1. Prepare smears of samples for subsequent staining to enable microscopic examination
  2. Prepare liquid films of specimens for direct observation for motility or cell structure
  3. Prepare samples to concentrate material for subsequent staining or microscopy
Prepare smears of samples for subsequent staining to enable microscopic examination

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare liquid films of specimens for direct observation for motility or cell structure

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare samples to concentrate material for subsequent staining or microscopy

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare pure cultures for microbiological work and aseptic applications

  1. Select culture media to maximise growth and optimise production of pure microbial cultures
  2. Inoculate media aseptically, applying techniques suitable for purpose of culture
  3. Incubate inoculated media in conditions to optimise targeted growth of organisms
Select culture media to maximise growth and optimise production of pure microbial cultures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Inoculate media aseptically, applying techniques suitable for purpose of culture

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Incubate inoculated media in conditions to optimise targeted growth of organisms

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Perform procedures that can assist in the identification of microorganisms

  1. Select staining techniques to demonstrate required cellular characteristics
  2. Stain prepared films to demonstrate diagnostically useful characteristics
  3. Inoculate and incubate media with pure cultures to assist in the biochemical and immunological identification of microorganisms
  4. Microscopically differentiate between, red blood cells, white blood cells, micro-organisms and epithelial cells
  5. Select and perform tests on pure cultures to determine the biochemical and immunological identification of microorganisms
  6. Perform antibiotic susceptibility testing
Select staining techniques to demonstrate required cellular characteristics

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Stain prepared films to demonstrate diagnostically useful characteristics

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Inoculate and incubate media with pure cultures to assist in the biochemical and immunological identification of microorganisms

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Microscopically differentiate between, red blood cells, white blood cells, micro-organisms and epithelial cells

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Select and perform tests on pure cultures to determine the biochemical and immunological identification of microorganisms

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Perform antibiotic susceptibility testing

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Estimate the number and/or size of micro-organisms in samples

  1. Count cells in samples
  2. Prepare serial dilutions of samples aseptically for culture and colony counting
  3. Indicate the appropriate dilution necessary to reliably count organisms in a serially diluted sample
  4. Count colonies for calculating number of viable organisms per unit volume
Count cells in samples

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare serial dilutions of samples aseptically for culture and colony counting

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Indicate the appropriate dilution necessary to reliably count organisms in a serially diluted sample

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Count colonies for calculating number of viable organisms per unit volume

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain records of laboratory work

  1. Record and/or check data in accordance with workplace procedures
  2. Follow procedures to ensure traceability of sample from receipt to reporting of results
Record and/or check data in accordance with workplace procedures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Follow procedures to ensure traceability of sample from receipt to reporting of results

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Assessed

Teacher: ___________________________________ Date: _________

Signature: ________________________________________________

Comments:

 

 

 

 

 

 

 

 

Instructions to Assessors

Required Skills and Knowledge